Journal
NANO LETTERS
Volume 10, Issue 9, Pages 3620-3627Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl101939e
Keywords
Viral assembly; nanotechnology; nanostructure; nanobiotechnology; nanomedicine; bacteriophage phi29; liposomes; ion channel; rectification; DNA packaging; nanomotors; membrane channel
Categories
Funding
- NIH Nanomedicine Development Center [PN2 EY 018230]
- [GM059944]
Ask authors/readers for more resources
Linear double-stranded DNA (dsDNA) viruses package their genome into a procapsid using an ATP-driven nanomotor. Here we report that bacteriophage phi29 DNA packaging motor exercises a one-way traffic property for dsDNA translocation from N-terminal entrance to C-terminal exit with a valve mechanism in DNA packaging, as demonstrated by voltage ramping, electrode polarity switching, and sedimentation force assessment. Without the use of gating control as Found in other biological channels, the observed single direction dsDNA transportation provides a novel system with a natural valve to control dsDNA loading and gene delivery in bioreactors, liposomes, or high throughput DNA sequencing apparatus.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available