Whole Genome Expression Analysis Reveals Differential Effects of TiO2 Nanotubes on Vascular Cells

The response of primary human endothelial (ECs) and vascular smooth muscle cells (VSMCs) to TiO2 nanotube arrays is studied through gene expression analysis. Microarrays revealed that nanotubes enhanced EC proliferation and motility, decreased VSMC proliferation, and decreased expression of molecules involved in inflammation and coagulation in both cell types. Networks generated front significantly affected genes suggest that cells may be sensing nanotopographical cues via pathways previously implicated in sensing shear stress.