Journal
NANO LETTERS
Volume 10, Issue 10, Pages 4249-4252Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl1027638
Keywords
Microscopy; resolution; chlorophyll; ground state depletion; nanoscopy; GSDIM
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Funding
- Deutsche Forschungsgemeinschaft
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We demonstrate far-field optical imaging at the nanoscale with unlabeled samples Subdiffraaion resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on off cycle to yield an image, a condition fulfilled by various biomolecules The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts
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