Journal
NANO LETTERS
Volume 10, Issue 11, Pages 4697-4701Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl102986v
Keywords
DNA; peptide nucleic acid; optical tweezers; microfluidic cross-slot; single-molecule; sequence-specific
Categories
Funding
- NIH [R21HG4342, GM32543, GMOZ1552]
- NSF
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The ability to strongly and sequence-specifically attach modifications such as fluorophores and haptens to individual double-stranded (ds) DNA molecules is critical to a variety of single-molecule experiments. We propose using modified peptide nucleic acids (PNAs) for this purpose and implement. them in two model single-molecule experiments where individual DNA molecules are manipulated via microfluidic flow and optical tweezers, respectively. We demonstrate that PNAs are versatile and robust sequence-specific tethers.
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