Journal
NANO LETTERS
Volume 10, Issue 8, Pages 2877-2883Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl100942p
Keywords
Silicon nanowire; streptavidin; CD4(+) T lymphocyte; cell separation
Categories
Funding
- Ministry of Education, Science and Technology [2009-0094032, 2009-0075729]
- KRIBB Research Initiative Program
- KICOS [K20704000007-09A0500-00710, K20601000002-09E0100-00210]
- Innovative Research Institute for Cell Therapy [A062260]
- National Nuclear RD program
- Korea Health Promotion Institute [A062260] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2009-0075729, 2009-00299, 2007-00107, 2009-0094032] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Silicon nanowires (SiNWs) offer promising inorganic nanostructures for biomedical application. Here, we report the development of a novel SiNW array designed for isolating primary CD4(+) T lymphocytes from the heterogeneous mixture of cell populations. Our system employed the specific high-affinity binding features of streptavidin (STR)-functionalizeci SiNW with biotin-labeled CD4(+) T lymphocytes. Fabricated SiNW arrays easily separated the CD4(+) T lymphocytes from the mouse whole splenocytes with over similar to 88% purity and demonstrated tight attachment to CD4(+) T lymphocytes by scanning electron microscopy. Thus, our STR-SiNW arrays provide a potential tool for specific cell separation and further present a possibility to be applied to the other area of biomedical applications.
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