Journal
NANO LETTERS
Volume 9, Issue 5, Pages 2021-2025Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl900283j
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Funding
- Australian Research Council [DP0772356]
- Australian Research Council [DP0772356] Funding Source: Australian Research Council
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Monitoring enzyme secretion in tissue culture has proved challenging because to date the activity cannot be continuously measured in situ. In this Letter, we present a solution using biopolymer loaded pholonic crystals of anodized silicon. Shifts in the optical response by proteolytic degradation of the biopolymer provide label-free sensing with unprecedented low detection limits (1 pg) and calculation of kinetic parameters. The enhancement in sensitivity relative to previous photonic crystal sensors constitutes a change in the sensing paradigm because here the entire pore space is responsive to the secreted enzyme rather than just the pore walls. In situ monitoring is demonstrated by detecting secretion of matrix metalloprotease 9 from stimulated human macrophages.
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