Journal
NANO LETTERS
Volume 9, Issue 6, Pages 2246-2250Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl9003464
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Funding
- National Natural Science Foundation of China [30700745, 90606028]
- Ministry of Health China [2008ZX10004-004]
- National Basic Research Program of China [2006CB933100]
- Nanoscience Project of China Academy of Sciences [kjcx2-sw-h12]
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Aiming to build a supersensitive and easily operable immunoassay, bifunctional protein nanowires were generated by seeding-induced self-assembling of the yeast amyloid protein Sup35p that genetically fused with protein G and an enzyme (methyl-parathion hydrolase, MPH), respectively. The protein nanowires possessed a high ratio of enzyme molecules to protein G, allowing a dramatic increase of the enzymatic signal when protein G was bound to an antibody target. As a result, a 100-fold enhancement of the sensitivity was obtained when applied in the detection of the Yersinia, pestis F1 antigen.
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