Sensing of UO22+ and Design of Logic Gates by the Application of Supramolecular Constructs of Ion-Dependent DNAzymes

Supramolecular constructs composed of ion-dependent DNAzymes and their substrates were used to develop DNAzyme cascades that enabled the sensitive detection Of UO22+ or the activation of logic gate operations. The supramolecular complex between the UO22+-dependent DNAzyme and its substrate leads, in the presence Of UO22+, to the cleavage of the substrate and to the release of the HRP-mimicking DNAzyme that enables the optical analysis Of UO22+ (detection limit 1 x 10(-9) M). Similarly, supramolecular complexes between the Mg2+- and UO22+- dependent DNAzymes and tailored substrates enables the design of the OR and AND logic gates, using Mg2+ and UO22+ as inputs.