Journal
MYCOSCIENCE
Volume 54, Issue 2, Pages 100-105Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.myc.2012.09.002
Keywords
Function; Over-expression
Categories
Funding
- National Natural Science Foundation of China [30970042]
- Fundamental Research Funds for the Central Universities [KYZ201121]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Chinese National Key Technology RD Program [2012BAD36B05]
- Science & Technology Pillar Program of Jiangsu Province, China [BE2011312]
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To better understand the functions of key genes involved in triterpene biosynthesis in Ganoderma lucidum, we cloned and characterized an acetyl coenzyme A acetyltransferase gene (AACT), designated Ganoderma lucidum AACT (Gl-aact). The open reading frame (ORF) of Gl-aact encoded a 417-amino acid polypeptide with a theoretical pI of 8.89. The Gl-AACT sequence was highly homologous to those of other fungal AACTs. Meanwhile, analysis of the Gl-aact expression profile revealed a positive correlation between Gl-aact transcript abundance and triterpene content changes during the development of G. lucidum. The treatment of mycelia with exogenous methyl jasmonate (MeJA) caused the accumulation of Gl-aact messenger RNA. Gl-aact over-expressing transformants were obtained by Agrobacterium tumefaciens-mediated transformation (ATMT), and triterpene production was observed to be increased when Gl-aact was over-expressed. Our results suggest that this enzyme may play an important role in triterpene biosynthesis. (C) 2012 The Mycological Society of Japan. Published by Elsevier B. V. All rights reserved.
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