Journal
MYCOPATHOLOGIA
Volume 171, Issue 1, Pages 11-21Publisher
SPRINGER
DOI: 10.1007/s11046-010-9342-1
Keywords
Candida glabrata; 5-Fluorocytosine; Resistance mechanisms; FUR1, FCY1 and FCY2 genes
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Funding
- Angers Loire Metropole
- Institut de Parasitologie de l'Ouest, Rennes, France
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Resistance to 5-fluorocytosine (5-FC) has been poorly investigated in the yeast Candida glabrata. This study was conducted on laboratory mutants obtained by exposure of a wild-type isolate to 5-FC. Based on their susceptibility to 5-fluorouracil (5-FU), two of these mutants were selected for further analysis of the molecular mechanisms of 5-FC resistance. One mutant, resistant to both compounds, exhibited a missense mutation in the gene coding the cytosine deaminase and a decrease in the expression level of the gene coding the uridine monophosphate pyrophosphorylase. The other mutant that showed a reduced susceptibility to 5-FC and 5-FU exhibited an overexpression of the genes coding the thymidylate synthase and a cytosine permease, associated with a missense mutation in the last gene. Thus, beside mutations in the FUR1 gene which represent the most common cause of resistance to 5-FC, other mechanisms may also occur in C. glabrata.
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