Journal
MYCOLOGICAL PROGRESS
Volume 11, Issue 2, Pages 395-401Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11557-011-0754-1
Keywords
Dendrobium officinale; Mycorrhizal fungi; Mycena; Anatomical characters; Epiphytic orchids
Categories
Funding
- National Natural Science Foundation of China [30770068]
- National High Technology Research and Development Program of China [2008AA09Z405]
- International Science and Technology Cooperation Projects of China [2009DFA32250]
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An endophytic fungus, F-23, was isolated from the roots of Dendrobium officinale Kimura et Migo, an endangered Chinese medicinal plant. The sequence of the ITS region indicated that the isolate belongs to the genus Mycena. After 4 months of inoculation, the root systems of D. officinale that were inoculated with F-23 fungus were much larger than the control's root systems. We also observed that the hyphae of F-23 penetrated the epidermal cells within the host's roots and spread from cell to cell. A large number of pelotons existed in the root cortical cells of D. officinale inoculated with F-23 fungus. Intracellular hyphae crossing through the host walls were also observed using SEM (scanning electron microscopy). In contrast, light microscopy and SEM showed that the transverse sections of the roots of control plants remained uncolonized. Therefore, the F-23 fungus can form mycorrhizal associations with the roots of its host plant, D. officinale, and enhance the growth of seedlings and roots. In brief, Mycena sp. was identified and shown to be a mycorrhizal fungus of the epiphytic orchid, D. officinale. This might be of potential use to the mass cultivation of D. officinale under artificial conditions.
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