Amatoxin and phallotoxin concentration in Amanita bisporigera spores

Even though amatoxins and phallotoxins have been well characterized in basidiocarps of Amanita species, to our knowledge no report of these toxins in spores of Amanitas has been published. Reversed phase HPLC was used to determine non-zero concentrations of a-amanitin (0.30 mg/g), and phallacidin (0.02 mg/g) in spores taken from white Amanita. sect. phalloideae species. We did not find significant amounts of phalloidin in Amanita spores. We also report concentrations of these toxins from pileus and stipe tissues that are similar to previously reported values, lending support to the hypothesis that toxin concentrations in spores are much less than in other basidiocarp tissues.