Journal
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
Volume 741, Issue 1-2, Pages 89-94Publisher
ELSEVIER
DOI: 10.1016/j.mrgentox.2011.11.004
Keywords
DBPs; DNA damage; SCGE assay; HepG2 cell; Genotoxicity
Funding
- National Key Technologies R&D Program of China [2006BAI19B02]
- National Natural Science Foundation of China (NNSFC) [81072264]
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Disinfection of drinking water reduces pathogenic infection, but generates disinfection by-products (DBPs) in drinking water. In this study, the effect of fifteen DBPs on DNA damage in human-derived hepatoma line (HepG2) was investigated by the single cell gel electrophoresis (SCGE) assay. These fifteen DBPs are: four trihalomethanes (THMs), six haloacetic acides (HAAs), three haloacetonitriles (HANs), 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), and chloral hydrate (CH). Based on the minimal effective concentration (MEC) at which DBPs induced significant increase in olive tail moment (OTM), the rank order of DNA-damaging potency is: bromodichloromethane (BDCM) > dibromochloromethane (DBCM) > tribromomethane (TBM) > trichloromethane (TCM) of the four THMs; iodoacetic acid (IA) > bromoacetic acid (BA) > dibromoacetic acid (DBA) > dichloracetic acid (DCA) > trichloroacetic acid (TCA) of the five HAAs; dibromoacetonitrile (DBN) approximate to dichloroacetonitrile (DCN) > trichloroacetonitrile (TCN) of the three HANs. The DNA damaging potency of MX and CH is similar to TCA and DCA, respectively. IA is the most genotoxic DBP in the fifteen DBPs, followed by BA. Chloroacetic acid (CA) is not genotoxic in this assay. Our findings indicated that HepG2/SCGE is a sensitive tool to evaluate the genotoxicity of DBPs and iodinated DBPs are more genotoxic than brominated DBPs, but chlorinated DBPs are less genotoxic than brominated DBPs. (C) 2011 Elsevier B.V. All rights reserved.
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