Journal
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
Volume 742, Issue 1-2, Pages 48-53Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mrgentox.2011.11.014
Keywords
Allium cepa; Zineb; Genotoxicity; Cytotoxicity; Microtubules
Funding
- National University of La Plata [11/N564, 11/N619]
- University of Buenos Aires [MDM-UBACyT X154]
- National Council for Scientific and Technological Research (CONICET) from Argentina [0106, 0744]
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Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the zineb-containing commercial formulation Azzurro (R) (70% zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro (R) (1 and 10 mu g/ml) induced a significant increase in the frequency of CAs (P < 0.05), and the higher concentration inhibited the MI (P < 0.05) compared to control values. The frequency of MN did not differ from control values at any concentration. Treatment with 1 mu g/ml Azzurro (R) induced a significant increase in the frequency of abnormal PPB (P < 0.01), MS (P < 0.001), and Phrag (P < 0.01) and, at 10 mu g/ml, enhancements in the frequencies of abnormal MS (P < 0.05) and Phrag (P < 0.05) were seen. A tubulin immunodetection assay showed that exposure to Azzurro (R) interferes with normal assembly of microtubule structures during mitosis. (C) 2011 Elsevier B.V. All rights reserved.
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