Journal
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
Volume 657, Issue 2, Pages 98-104Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mrgentox.2008.08.001
Keywords
Aflatoxin B-1; Pheophorbide; Glutathione S-transferase; GST; Chlorophyllide; Chlorophyll derivatives
Funding
- Chang-Gung Memorial Hospital [CMRPF32003]
Ask authors/readers for more resources
The inhibitory effects of four chlorophyll derivatives (chlorophyllide [Chlide] a and b and pheophorbide [Pho] a and b) on aflatoxin B, (AFB(1))-DNA adduct formation, and on the modulation of hepatic glutathione S-transferase (GST) were evaluated in murine hepatoma (Hepa-1) cells. Enzyme-linked immunosorbent assay showed that pretreatment with Chlide or Pho significantly reduced the formation of AFB(1)-DNA adducts, and that Pho was the most potent inhibitor. However, wash-out prior to adding AFB(1) totally eliminated inhibition by Childe and partially eliminated inhibition by Pho. indicating that the inhibitory effect of Chlide, and to some extent Pho, was mediated through direct trapping of AFB(1). Furthermore, spectrophotometric analysis showed that Pho treatment could increase GST activity in Hepa-1 cells. These observations indicate that the chlorophyll derivatives studied may attenuate AFB(1)-induced DNA damage in the Hepa-1 cell by direct trapping of AFB(1). Pho provided additional protection not only by direct trapping, but also by increasing GST activity against hepatic AFB(1) metabolites. (c) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available