Journal
MUSCLE & NERVE
Volume 42, Issue 2, Pages 268-270Publisher
WILEY-BLACKWELL
DOI: 10.1002/mus.21700
Keywords
genotyping; mouse dystrophin gene; mdx mice
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Funding
- CJ Martin postdoctoral fellowship
- MDA
- NIH [AR044533, NS046788]
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Currently available polymerase chain reaction (PCR) genotyping methods for point mutations in the mouse dystrophin gene can lead to false positives and result in wasted time and money due to breeding or treating the wrong mice. Here we describe a simple and accurate method for sequencing the point mutations in mdx, mdx(4cv), and mdx(5cv) mice. This method clearly distinguishes between wildtype, heterozygous, and mutant transcripts, and thereby time and money can be saved by avoiding false positives. Muscle Nerve 42: 268-270, 2010
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