Journal
MUSCLE & NERVE
Volume 40, Issue 3, Pages 455-465Publisher
WILEY
DOI: 10.1002/mus.21369
Keywords
active satellite cell; NCAM; muscle fiber; myonucleus; Pax7
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Funding
- Lundbeck Foundation
- Danish National Research Foundation
- Danish Health Science Research Board
- Swedish National Center for Research in Sports
- Mid the Loo and Hans Osterman Foundation
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The primary aim of our study was to validate the assessment of myonuclear and satellite cell number in biopsies from human skeletal muscle. We found that 25 type I and 25 type II fibers are sufficient to estimate the mean number of myonuclei per fiber. In contrast, the assessment of satellite cells improved when more fibers were included. Second, we report that small differences in counting satellite cells using CD56 and Pax7 antibodies can be attributed to the different staining profiles. Third, we provide support for the use of Ki67 in evaluating the proportion of active satellite cells. We observed very few (up to 1.3%) active satellite cells in healthy adult skeletal muscle at rest, but they increased significantly (up to 7-fold) following muscle activity. This study provides valuable tools to assess the behavior of satellite cells, both in pathological conditions and in response to physiological stimuli. Muscle Nerve 40: 455-465, 2009
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