Journal
MUCOSAL IMMUNOLOGY
Volume 2, Issue 2, Pages 156-165Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/mi.2008.71
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Funding
- BBSRC
- Biotechnology and Biological Sciences Research Council [BB/D012643/2] Funding Source: researchfish
- BBSRC [BB/D012643/2] Funding Source: UKRI
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Steady-state dendritic cells (DCs) migrating in the lymph from the intestine induce tolerance to harmless intestinal antigens, preventing inflammatory responses. To determine if such DCs are inherently tolerogenic we collected intestinal lymph DCs (L-DCs) by cannulation of the thoracic duct of rats after mesenteric lymphadenectomy, and examined their capacity to activate naive CD4(+) lymphocytes in an allogeneic mixed leucocyte reaction. L-DCs stimulated strong proliferative responses, induced secretion of inflammatory cytokines including interferon-gamma, and induced FoxP3-positive lymphocytes to divide. To determine if the activated CD4(+) T cells had been tolerized, they were rested and restimulated with irradiated splenocytes. The restimulated CD4(+) T cells again proliferated and secreted inflammatory cytokines. These data demonstrate that the DCs, which migrate from the intestine in the steady state, are paradoxically able to induce strong inflammatory responses from naive T cells, despite their role in the maintenance of oral tolerance.
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