Journal
AQUATIC TOXICOLOGY
Volume 168, Issue -, Pages 72-77Publisher
ELSEVIER
DOI: 10.1016/j.aquatox.2015.09.013
Keywords
Nano-TiO2; CdCl2; Dicentrarchus labrax; Genotoxicity; DNA damage; Micronuclei
Categories
Funding
- Italian Ministry of Research Marine ecotoxicology of nanomaterials: toxicity and bioaccumulation of nano titanium dioxide in edible species in the presence of metals and dioxin [PRIN2009FHHP2W]
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Due to the large production and growing use of titanium dioxide nanoparticles (n-TiO2), their release in the marine environment and their potential interaction with existing toxic contaminants represent a growing concern for biota. Different end-points of genotoxicity were investigated in the European sea bass Dicentrarchus labrax exposed to n-TiO2 (1 mg L-1) either alone and combined with CdCl2 (0.1 mg L-1) for 7 days. DNA primary damage (comet assay), apoptotic cells (diffusion assay), occurrence of micronuclei and nuclear abnormalities (cytome assay) were assessed in peripheral erythrocytes and genomic stability (random amplified polymorphism DNA-PCR, RAPD assay) in muscle tissue. Results showed that genome template stability was reduced after CdCl2 and n-TiO2 exposure. Exposure to n-TiO2 alone was responsible for chromosomal alteration but ineffective in terms of DNA damage; while the opposite was observed in CdCl2 exposed specimens. Co-exposure apparently prevents the chromosomal damage and leads to a partial recovery of the genome template stability. (C) 2015 Elsevier B.V. All rights reserved.
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