Journal
AQUATIC TOXICOLOGY
Volume 165, Issue -, Pages 64-72Publisher
ELSEVIER
DOI: 10.1016/j.aquatox.2015.05.012
Keywords
Microalga; Atrazine; Cytotoxicity; RNA-Seq; Flow cytometry
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Funding
- Spanish Ministerio de Economia y Competitividad [CGL2010-15993/BOS]
- Xunta de Galicia (Spain)
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Chlamydomonas reinhardtii cells were exposed to a sublethal concentration of the widespread herbicide atrazine for 3 and 24 h. Physiological parameters related to cellular energy status, such as cellular activity and mitochondrial and cytoplasmic membrane potentials, monitored by flow cytometry, were altered in microalgal cells exposed to 0.25 mu M of atrazine. Transcriptomic analyses, carried out by RNA-Seq technique, displayed 12 differentially expressed genes between control cultures and atrazine-exposed cultures at both tested times. Many cellular processes were affected, but the most significant changes were observed in genes implicated in amino acid catabolism and respiratory cellular process. Obtained results suggest that photosynthesis inhibition by atrazine leads cells to get energy through a heterotrophic metabolism to maintain their viability. (C) 2015 Elsevier B.V. All rights reserved.
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