Journal
MOLECULES AND CELLS
Volume 29, Issue 5, Pages 527-531Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
DOI: 10.1007/s10059-010-0065-4
Keywords
Apoptosis; Fas; heat shock factor 1 (HSF1)
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Funding
- National Natural Science Fund of China [30600274]
- Guangzhou Science and Technology Board of China [2006F1-C0171]
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In mammalian cells, stress-induced expression of heat shock protein is controlled by heat shock factor 1 (HSF1). However, HSF1 functions as a regulator of additional genes. In this study, we observed that heat treatment effectively induced expression of Fas. Using bioinformatics, a high affinity and functional HSF1-binding element within the -1996/-1985 oligonucleotide of the 5'-flanking region of the Fas gene was found, and was determined by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. Exogenous expression of a constitutively activative HSF1, induced Fas gene transcription and protein synthesis in the absence of heat stress. Moreover, RNA interference-mediated HSF1 gene-silencing attenuated Fas expression in a heat-induced model. Our results suggested that HSF1 is an important transcription factor of Fas gene.
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