Journal
MOLECULES
Volume 23, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/molecules23071815
Keywords
Brassica napus; yellow seed; phenolic components; flavonoids; antioxidation
Funding
- National Natural Science Foundations [31330057, 31771825, 31401414]
- National Key Research and Development Program of China [2016YFD0101000, 2016YFD0102000]
- China Postdoctoral Science Foundation [2015T80591, 2014M561719]
- Jiangsu Postdoctoral Science Foundation [1401078B]
- Natural Science Foundation of Jiangsu Province [BK20140478]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYLX16_1386, XKYCX17_066]
- Undergraduate Training Program for Innovation and Entrepreneurship [x20170813]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Yangzhou University for Excellent Talent Support Program
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Brassica napus L. is rich in phenolic components and it has natural antioxidant characteristics which are important to human health. In the present study, the total phenolic and flavonoid contents of developing seeds of yellow- and black-seeded B. napus were compared. Both phenolic and flavonoid contents were significantly higher at 5 weeks after flowering (WAF) in black seeds (6.44 +/- 0.97 mg EE/g phenolics and 3.78 +/- 0.05 mg EE/g flavonoids) than yellow seeds (2.80 +/- 0.13 mg/g phenolics and 0.83 +/- 0.01 mg/g flavonoids). HPLC-DAD-ESI/MS analysis revealed different content of 56 phenolic components between yellow and black-seeded B. napus, including kaempferol-3-O-glucoside, isorhamnetin-3-O-glucoside, quercetin-3-O-sophoroside, procyanidin B2 ([DP 2]), which were significantly reduced in yellow seeds compared with black seeds. Applying the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical assay, we found maximum clearance of DPPH and ABTS in the late developmental stages of yellow and black seeds. Additionally, the ferric reducing antioxidant power (FRAP) value maximized at 5 WAF in black seeds (432.52 +/- 69.98 mol Fe (II)/g DW) and 6 WAF in yellow seeds (274.08 +/- 2.40 mol Fe (II)/g DW). Generally, antioxidant ability was significantly reduced in yellow-seeded B. napus compared to black rapeseed, and positive correlations between antioxidation and flavonoid content were found in both yellow- and black-seeded B. napus.
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