Journal
MOLECULES
Volume 19, Issue 6, Pages 8373-8386Publisher
MDPI
DOI: 10.3390/molecules19068373
Keywords
Castanea mollissima Blume; polyphenols; alpha-glucosidase; diabetic; ellagitannins
Funding
- Fundamental Research Funds for the Central Universities, China [YX2012-02]
- 111 Project [B13007]
Ask authors/readers for more resources
Polyphenol extracts from the burs of Castanea mollissima Blume (CMPE) exhibited potential antioxidant and hypoglycemic activities. The alpha-glucosidase inhibitory activities of CMPE were assessed as a means of elucidating the mechanism behind its hypoglycemic activities. In vitro studies showed that CMPE significantly inhibited both yeast alpha-glucosidase, through a noncompetitive mode with an IC50 of 0.33 mu g/mL, and rat intestinal alpha-glucosidase. In vivo studies revealed that oral administration of CMPE at doses of 600 mg/kg significantly reduced postprandial blood glucose levels by 27.2% in normal rats following sucrose challenges. Gel permeation chromatography revealed that CMPE exhibited typical characteristics of high-molecular-mass polymers with mean (Mn) and weight (Mw) average molecular weights of 35.4 and 50.7 kDa, respectively, and a polydispersity (Mw/Mn) of 1.432. Acid hydrolysis analysis indicated the presence of ellagitannins. These data suggest that CMPE, enriched with ellagitannins, would be an efficacious dietary supplement for diabetes management through the inhibition of alpha-glucosidase.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available