Journal
MOLECULES
Volume 19, Issue 9, Pages 15042-15057Publisher
MDPI AG
DOI: 10.3390/molecules190915042
Keywords
Micromelum falcatum; Rutaceae; 7-oxygenated coumarins; LC-MS; LTQ-Orbitrap; microfalcrin; microcoumaririn; micromelosidester; anti-inflammatory activity; NF-kappa B; NO production
Funding
- INsPiRE project [EU-FP7-REGPOT-2011]
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Micromelum falcatum, a medicinal plant of the Rutaceae family, has been used in the Traditional Chinese Medicine (TCM) mainly against colds and rheumatoid arthritis. Despite its traditional use the association of its constituents with possible anti-inflammatory activity has not been explored. During this study, a rapid UPLC-ESI(+)-HRMS method was developed for the profiling of M. falcatum leave extracts and the targeted isolation of coumarin constituents. Based on chromatographic, spectroscopic and spectrometric features several 7-oxygenated coumarin derivatives were detected. After targeted isolation, eight coumarins, among them three new natural products, namely microfalcrin, microcoumaririn and micromelosidester, were purified using semi-preparative HPLC and unambiguously identified by 1 and 2D NMR. Furthermore, important spectrometric characteristics were revealed based on the HRMS and HRMS/MS spectra of the isolated 7-oxygenated coumarins facilitating their identification in complex mixtures. Finally, the anti-inflammatory properties of the extracts and representative compounds were evaluated by measuring the inhibition of the pro-inflammatory mediator NF-kappa B induction and nitric oxide (NO) production.
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