Journal
MOLECULES
Volume 19, Issue 6, Pages 7568-7580Publisher
MDPI AG
DOI: 10.3390/molecules19067568
Keywords
tea seed pomace; saponin; LC-ESI-IT-TOF/MS; anti-fungal activity
Funding
- National Natural Science Foundation of China [31301601]
- National Science and Technology Pillar Program [2009BADB1B10]
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A method using LC-ESI-IT-TOF/MS and LC/UV-ELSD was established to qualitatively analyze triterpene saponins obtained from the tea seed pomace ( Camellia oleifera Abel). In addition, the quantitative analysis of oleiferasaponin A(1) using LC/UV was developed. The purified total saponins did not exhibit any inhibitory effects at concentrations ranging from 0.1 to 10 mg/mL against the tested bacteria, except for Staphyloccocus aureus and Escherichia coli. By contrast, higher inhibitory activity was seen against the tested fungi, especially against Bipolaris maydis. Following treatment with an MIC value of 250 mu g/mL for 24 h, the mycelial morphology was markedly shriveled in appearance or showed flattened and empty hyphae, with fractured cell walls, ruptured plasmalemma and cytoplasmic coagulation or leakage. These structural changes hindered the growth of mycelia.
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