Journal
MOLECULES
Volume 19, Issue 7, Pages 9850-9863Publisher
MDPI
DOI: 10.3390/molecules19079850
Keywords
polyphosphazene hydrogel; swelling; lipase; entrapment; activity
Funding
- Fundamental Research Funds for the Central Universities [2013QNA4090]
- National Natural Science Foundation of China [21274126]
- National Twelfth Five Year Plan for Science & Technology Support of China [2012BAI08B01]
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We report on the synthesis and application of a new hydrogel based on a methacrylate substituted polyphosphazene. Through ring-opening polymerization and nucleophilic substitution, poly[bis(methacrylate) phosphazene] (PBMAP) was successfully synthesized from hexachlorocyclotriphosphazene. By adding PBMAP to methacrylic acid solution and then treating with UV light, we could obtain a cross-linked polyphosphazene network, which showed an ultra-high absorbency for distilled water. Lipase from Candida rugosa was used as the model lipase for entrapment immobilization in the hydrogel. The influence of methacrylic acid concentration on immobilization efficiency was studied. Results showed that enzyme loading reached a maximum of 24.02 mg/g with an activity retention of 67.25% when the methacrylic acid concentration was 20% (w/w).
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