Journal
MOLECULES
Volume 18, Issue 1, Pages 574-587Publisher
MDPI
DOI: 10.3390/molecules18010574
Keywords
epigallocatechin-3-gallate; photodegradation; photostabilizers; topical emulsions; in vitro antioxidant activity; co-antioxidants
Ask authors/readers for more resources
The catechin (-)-epigallocatechin-3-gallate (EGCG) exhibits high antioxidant activity and it has been reported to provide protection of the skin against damage induced by solar UV radiation. However, EGCG is highly unstable under sunlight. The present study aimed to compare the effectiveness of the co-antioxidant agents vitamin E, butylated hydroxytoluene, vitamin C and alpha-lipoic acid for their potential to protect the catechin from photochemical degradation. Model creams (oil-in-water emulsions) containing EGCG (1%, w/w) alone or combined with equimolar concentrations of co-antioxidant were exposed to a solar simulator at an irradiance corresponding to natural sunlight. Photodegradation was evaluated by HPLC-UV and HPLC-ESI-MS/MS. Addition of the co-antioxidants vitamin C and alpha-lipoic acid to the formulation significantly reduced the light-induced decomposition of EGCG from 76.9 +/- 4.6% to 20.4 +/- 2.7% and 12.6 +/- 1.6%, respectively. Conversely, butylated hydroxytoluene had no effect (EGCG loss, 78.1 +/- 4.6%) and vitamin E enhanced the EGCG photolysis to 84.5 +/- 3.4%. The functional stability of the catechin in the creams exposed to the solar simulator was also evaluated by measuring the in vitro antioxidant activity. Following irradiation, the reduction of the EGCG formulation antioxidant power was lower (21.8%) than the extent of degradation (76.9%), suggesting the formation of photoproducts with antioxidant properties. The influence of the examined co-antioxidants on the functional stability of the catechin under simulated sunlight paralleled that measured for the EGCG photodecomposition, alpha-lipoic acid exerting the greatest stabilising effect (antioxidant activity decrease, 1.4%). These results demonstrated that alpha-lipoic acid is an effective co-antioxidant agent for the stabilization of EGCG in dermatological products for skin photoprotection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available