Journal
MOLECULES
Volume 18, Issue 1, Pages 1101-1110Publisher
MDPI
DOI: 10.3390/molecules18011101
Keywords
black soybean sprouts; antioxidant; ultrasonic assisted extraction; aqueous extraction; response surface methodology
Funding
- Shanghai Jahwa United Co. Ltd
- Ph. D. Science Research Foundation of Jiangnan University
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Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of antioxidants from black soybean (Glycine max var) sprouts. Three influencing factors: liquid-solid ratio, period of ultrasonic assisted extraction and extraction temperature were investigated in the ultrasonic aqueous extraction. Then Response Surface Methodology (RSM) was applied to optimize the extraction process focused on DPPH radical-scavenging capacity of the antioxidants with respect to the above influencing factors. The best combination of each significant factor was determined by RSM design and optimum pretreatment conditions for maximum radical-scavenging capacity were established to be liquid-solid ratio of 29.19: 1, extraction time of 32.13 min, and extraction temperature of 30 degrees C. Under these conditions, 67.60% of DPPH radical-scavenging capacity was observed experimentally, similar to the theoretical prediction of 66.36%.
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