4.6 Article

A Sandwich HIV p24 Amperometric Immunosensor Based on a Direct Gold Electroplating-Modified Electrode

Journal

MOLECULES
Volume 17, Issue 5, Pages 5988-6000

Publisher

MDPI AG
DOI: 10.3390/molecules17055988

Keywords

HIV; p24; sandwich amperometric immunosensor; direct electroplating

Funding

  1. National Natural Science Foundation of China [20805024]
  2. Science and Technology Program of Guangdong Province [2010A030300006, 2008A050200006]
  3. Science and Technology Project of Ningbo [2009D10010, 2011A610018]
  4. Wong K.C megna foundation in Ningbo University
  5. Natural Science Foundation of Guangdong Province [S2011010003840]

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Acquired immune deficiency syndrome (AIDS) is a severe communicable immune deficiency disease caused by the human immune deficiency virus (HIV). The analysis laboratory diagnosis of HIV infection is a crucial aspect of controlling AIDS. The p24 antigen, the HIV-1 capsid protein, is of considerable diagnostic interest because it is detectable several days earlier than host-generated HIV antibodies following HIV exposure. We present herein a new sandwich HIV p24 immunosensor based on directly electroplating an electrode surface with gold nanoparticles using chronoamperometry, which greatly increased the conductivity and reversibility of the electrode. Under optimum conditions, the electrochemical signal showed a linear relationship with the concentration of p24, ranging from 0.01 ng/mL to 100 ng/mL (R > 0.99), and the detection limit was 0.008 ng/mL. Compared with ELISA, this method increased the sensitivity by more than two orders of magnitude (the sensitivity of ELISA for p24 is about 1 ng/mL). This immunosensor may be broadly applied to clinical samples, being distinguished by its ease of use, mild reaction conditions, guaranteed reproducibility, and good anti-interference ability.

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