Journal
MOLECULES
Volume 17, Issue 3, Pages 2474-2490Publisher
MDPI
DOI: 10.3390/molecules17032474
Keywords
optically-encoded bead; fluorescence; quantum dots; surface-enhanced Raman scattering (SERS); bead-based assay; label-free detection; high-throughput screening
Funding
- National Research Foundation (NRF)
- Korean government (MEST) [2011-0029945]
- Pioneer Research Center through the National Research Foundation of Korea
- Ministry of Education, Science and Technology [20110021021]
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Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.
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