Journal
MOLECULES
Volume 17, Issue 4, Pages 3761-3773Publisher
MDPI
DOI: 10.3390/molecules17043761
Keywords
endophytic fungus; Berkleasmium sp Dzf12; spirobisnaphthalene; palmarumycin C-12; palmarumycin C-13; crude oligosaccharide; polysaccharide; elicitation; Dioscorea zingiberensis
Funding
- National Natural Science Foundation of China [31071710]
- program for Changjiang Scholars and Innovative Research Team in University of China [IRT1042]
- Natural Science Foundation of Beijing [6092015]
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Three crude oligosaccharides were respectively prepared by acid hydrolysis of three polysaccharides, which were water-extracted polysaccharide (WEP), sodium hydroxide-extracted polysaccharide (SEP) and acid-extracted polysaccharide (AEP) from the rhizomes of Dioscorea zingiberensis. Among the three oligosaccharides, the crude oligosaccharide prepared by acid hydrolysis of WEP was found to be the most efficient elicitor to enhance the production of palmarumycins C-12 and C-13 in liquid culture of endophytic fungus Berkleasmium sp. Dzf12. When OW was applied to the medium at 300 mg/L on day 3 of culture, the maximal yields of palmarumycin C-12 (87.96 mg/L) and palmarumycin C-13 (422.28 mg/L) were achieved on day 15 of culture, which were 9.83 and3.24-fold in comparison with those (8.95 and 130.43 mg/L) of control, respectively. The results indicate that addition of the oligosaccharides from the host plant D. zingiberensis should be an effective strategy for enhancing production of palmarumycins C-12 and C-13 in liquid culture of endophytic fungus Berkleasmium sp. Dzf12.
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