Journal
MOLECULES
Volume 16, Issue 9, Pages 7949-7957Publisher
MDPI AG
DOI: 10.3390/molecules16097949
Keywords
Isodon rubescens; high-speed counter-current chromatography (HSCCC); oridonin
Funding
- Natural Science Foundation of Heilongjiang Province [LC2009C12]
- Heilongjiang Province Department of Health [2009-259]
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Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography (HPLC). A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, (1)H-NMR and (13)C-NMR.
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