Journal
MOLECULES
Volume 16, Issue 2, Pages 1454-1470Publisher
MDPI AG
DOI: 10.3390/molecules16021454
Keywords
Micromeria croatica; Micromeria juliana; Micromeria thymifolia; antioxidant activity; polyphenols; rosmarinic acid
Funding
- Ministry of Science, Education and Sports of Republic of Croatia [006-0061117-1238, 006-1340227-1248]
- Bilateral Croatian-Hungarian Project [HR 28/2008]
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Antioxidant activities of three selected Micromeria species growing in Croatia (M. croatica, M. juliana and M. thymifolia) were evaluated using five different antioxidant assays, in comparison with plant polyphenolic constituents and reference antioxidants. All studied ethanolic extracts exhibited considerable activity to scavenge DPPH and hydroxyl free radicals, reducing power, iron chelating ability and total antioxidant capacity in the order: M. croatica > M. juliana > M. thymifolia. Total polyphenol (9.69-13.66%), phenolic acid (5.26-6.84%), flavonoid (0.01-0.09%) and tannin (3.07-6.48%) contents in dried plant samples were determined spectrophotometrically. A strong positive correlation between antioxidant activities and contents of phenolic acids and tannins was found, indicating their responsibility for effectiveness of tested plants. Our findings established Micromeria species as a rich source of antioxidant polyphenols, especially the endemic M. croatica.
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