Journal
MOLECULES
Volume 15, Issue 8, Pages 5692-5707Publisher
MDPI AG
DOI: 10.3390/molecules15085692
Keywords
solid-phase synthesis; oligonucleotides; DNA; thiol; oligonucleotide conjugates
Funding
- EECC [NEST-ADV028669, FP7-NMP-213382-2]
- Spanish Ministry of Education [BFU2007-63287]
- Generalitat de Catalunya [2009/SGR/208]
- VI National RDi Plan
- Instituto de Salud Carlos III
- Iniciativa Ingenio
- Consolider
- CIBER Actions
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An efficient route for the synthesis of the phosphoramidite derivative of 5-methylcytosine bearing a tert-butylsulfanyl group protected thiol is described. This building block is used for the preparation of oligonucleotides carrying a thiol group at the nucleobase at the internal position of a DNA sequence. The resulting thiolated oligonucleotides are useful intermediates to generate oligonucleotide conjugates carrying molecules of interest at internal positions of a DNA sequence.
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