Journal
MOLECULES
Volume 15, Issue 5, Pages 3556-3566Publisher
MDPI
DOI: 10.3390/molecules15053556
Keywords
Corydalis yanhusuo; alkaloids; aromatase; ultrafiltration LC-MS
Funding
- Analysis and Detection Foundation of Science and Technology Department of Zhejiang Province [2008F017]
- Education Department of Zhejiang Province
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Aromatase represents an important target for the treatment of hormone-dependent breast cancer. In the present study, nine alkaloids from the dichloromethane extract of Corydalis yanhusuo were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS) and tested for their aromatase binding activities using an ultrafiltration LC-MS method by investigating the differences of peak areas of compounds before and after incubations with aromatase. It was demonstrated that the quaternary protoberberine alkaloids and the tertiary protoberberine alkaloids exhibited potent aromatase binding activities. The quaternary ammonium group and the methyl group at C-13 position of tertiary protoberberine alkaloids might be necessary for the activity. The findings should provide guidance for the discovery of potential aromatase inhibitors from natural products.
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