Journal
MOLECULES
Volume 15, Issue 11, Pages 7547-7557Publisher
MDPI
DOI: 10.3390/molecules15117547
Keywords
Curcuma; TLC; DPPH; GC-MS; Free radical scavenging activity
Funding
- Science and Technology Development Fund of Macau [028/2007/A2]
- Ministry of Science and Technology of The People's Republic of China [2009ZX09308-005]
- China Post-doctoral Scientific Fund [20100470]
- China Academy of Chinese Medical Sciences [ZZ20090111]
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The sesquiterpenoids are one of major groups of antioxidants in Curcuma besides curcuminoids. However, the real substances contributing to the antioxidant activity are still unknown. In this paper, the antioxidant activity of sesquiterpenoids in four species and two essential oils from Curcuma genus was determined and compared based on TLC separation and DPPH bioautography assay. Their antioxidant capacities were quantitatively evaluated using densitometry with detection at 530 nm (lambda(reference) = 800 nm) using vitamin C as reference. The results showed that Curcuma longa rhizomes had the highest antioxidant capacity while C. phaeocaulis presented the lowest one among the four species of Curcuma. Moreover, essential oil of C. wenyujin showed higher antioxidant potential than that of C. longa. The main TLC bands with antioxidant activity of the four species of Curcuma were collected and characterized using GC-MS, and thus curzerene, furanodiene, alpha-turmerone, beta-turmerone and beta-sesquiphellandrene were determined as major sesquiterpenoids with antioxidant activity in Curcuma.
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