Journal
MOLECULAR SYSTEMS BIOLOGY
Volume 6, Issue -, Pages -Publisher
WILEY
DOI: 10.1038/msb.2010.79
Keywords
chromosome conformation; chromatin; computational biology; genome architecture; networks
Categories
Funding
- EMBO
- ANR [ASTF 277-2009, ANR-09-PIRI-0024]
- EC [217141]
- Queens' College, Cambridge
- Agence Nationale de la Recherche (ANR) [ANR-09-PIRI-0024] Funding Source: Agence Nationale de la Recherche (ANR)
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A prime goal in systems biology is the comprehensive use of existing high-throughput genomic datasets to gain a better understanding of chromatin organization and genome function. In this report, we use chromatin immunoprecipitation (ChIP) data that map protein-binding sites on the genome, and Hi-C data that map interactions between DNA fragments in the genome in an integrative approach. We first reanalyzed the contact map of the human genome as determined with Hi-C and found that long-range interactions are highly nonrandom; the same DNA fragments are often found interacting together. We then show using ChIP data that these interactions can be explained by the action of the CCCTC-binding factor (CTCF). These CTCF-mediated interactions are found both within chromosomes and in between different chromosomes. This makes CTCF a major organizer of both the structure of the chromosomal fiber within each individual chromosome and of the chromosome territories within the cell nucleus. Molecular Systems Biology 6: 426; published online 2 November 2010; doi:10.1038/msb.2010.79
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