Journal
MOLECULAR SYSTEMS BIOLOGY
Volume 5, Issue -, Pages -Publisher
WILEY
DOI: 10.1038/msb.2009.70
Keywords
a higher-order singular value decomposition (HOSVD); cell cycle; DNA replication origin licensing and firing; DNA microarrays; mRNA expression
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Funding
- Cancer Research UK
- National Human Genome Research Institute [HG004302]
- National Science Foundation [DMS-0847173]
- Division Of Mathematical Sciences
- Direct For Mathematical & Physical Scien [0847173] Funding Source: National Science Foundation
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This report provides a global view of how gene expression is affected by DNA replication. We analyzed synchronized cultures of Saccharomyces cerevisiae under conditions that prevent DNA replication initiation without delaying cell cycle progression. We use a higher-order singular value decomposition to integrate the global mRNA expression measured in the multiple time courses, detect and remove experimental artifacts and identify significant combinations of patterns of expression variation across the genes, time points and conditions. We find that, first, similar to 88% of the global mRNA expression is independent of DNA replication. Second, the requirement of DNA replication for efficient histone gene expression is independent of conditions that elicit DNA damage checkpoint responses. Third, origin licensing decreases the expression of genes with origins near their 30 ends, revealing that downstream origins can regulate the expression of upstream genes. This confirms previous predictions from mathematical modeling of a global causal coordination between DNA replication origin activity and mRNA expression, and shows that mathematical modeling of DNA microarray data can be used to correctly predict previously unknown biological modes of regulation. Molecular Systems Biology 5: 312; published online 13 October 2009; doi:10.1038/msb.2009.70
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