A primer on the fundamental principles of light microscopy: Optimizing magnification, resolution, and contrast

The light microscope is an indispensable tool in the study of living organisms. Most biologists are familiar with microscopes, perhaps being first introduced to the wonders of the world of small things at a very early age. Yet, few fully comprehend the nature of microscopy and the basis of its utility. This review (re)-introduces the concepts of magnification, resolution, and contrast, and explores how they are intimately related and necessary for effective microscopy. Mol. Reprod. Dev. 82: 502-507, 2015. (c) 2014 Wiley Periodicals, Inc.