Journal
MOLECULAR REPRODUCTION AND DEVELOPMENT
Volume 82, Issue 7-8, Pages 605-618Publisher
WILEY
DOI: 10.1002/mrd.22258
Keywords
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Funding
- Howard Hughes Medical Institute
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In vivo imaging applications typically require carefully balancing conflicting parameters. Often it is necessary to achieve high imaging speed, low photo-bleaching, and photo-toxicity, good three-dimensional resolution, high signal-to-noise ratio, and excellent physical coverage at the same time. Light-sheet microscopy provides good performance in all of these categories, and is thus emerging as a particularly powerful live imaging method for the life sciences. We see an outstanding potential for applying light-sheet microscopy to the study of development and function of the early nervous system in vertebrates and higher invertebrates. Here, we review state-of-the-art approaches to live imaging of early development, and show how the unique capabilities of light-sheet microscopy can further advance our understanding of the development and function of the nervous system. We discuss key considerations in the design of light-sheet microscopy experiments, including sample preparation and fluorescent marker strategies, and provide an outlook for future directions in the field. Mol. Reprod. Dev. 82: 605-618, 2015. (c) 2013 Wiley Periodicals, Inc.
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