Journal
MOLECULAR REPRODUCTION AND DEVELOPMENT
Volume 77, Issue 7, Pages 640-647Publisher
WILEY
DOI: 10.1002/mrd.21198
Keywords
-
Funding
- JSPS [18390022, 21390019]
- MEXT [19044019, 21025015]
- Grants-in-Aid for Scientific Research [21112002, 18390022, 21112001, 21390019, 21025015, 19044019] Funding Source: KAKEN
Ask authors/readers for more resources
The ubiquitin proteasome system is known to play a key role in fertilization in ascidians, sea urchins, and mammals. To obtain insights into the ubiquitin-conjugating enzymes (Ube2) involved in reproductive systems, we systematically explored Ube2 enzymes expressed in the testis of the ascidian Ciona intestinalis. Here, we report cDNA cloning and characterization of a novel type of Ube2r (Ci0100152677) that is capable of making a thiolester bond with ubiquitin. Northern analysis, whole-mount in situ hybridization and immunocytochemistry indicate that this enzyme is exclusively expressed in the testis, mainly in the germ cells during the late stage of spermatogenesis, and is localized in the sperm head and tail, suggesting possible participation in fertilization or spermatogenesis/spermiogenesis. Mol. Reprod. Dev. 77: 640-647, 2010. (C) 2010 Wiley-Liss, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available