Journal
MOLECULAR PLANT-MICROBE INTERACTIONS
Volume 27, Issue 3, Pages 286-295Publisher
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI-05-13-0154-FI
Keywords
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Funding
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/G042960/1, BB/J004553/1]
- Biotechnology and Biological Sciences Research Council (BBSRC) (as part of the consortium ERA-PG PRR-CROP) [BB/G024936/1]
- BBSRC doctoral training grant
- Gatsby Charitable Foundation
- Biotechnology and Biological Sciences Research Council [BBS/E/J/000C0664, BB/G024936/1, BB/G024960/1] Funding Source: researchfish
- BBSRC [BB/G024960/1, BB/G024936/1, BBS/E/J/000C0664] Funding Source: UKRI
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The first layer of active defense in plants is based on the perception of pathogen-associated molecular patterns (PAMPs) leading to PAMP-triggered immunity (PTI). PTI is increasingly being investigated in crop plants, where it may have potential to provide durable disease resistance in the field. Limiting this work, however, is an absence of reliable bioassays to investigate PAMP responses in some species. Here, we present a series of methods to investigate PTI in Brassica napus. The assays allow measuring early responses such as the oxidative burst, mitogen-activated protein kinase phosphorylation, and PAMP-induced marker gene expression. Illumina-based RNA sequencing analysis produced a genome-wide survey of transcriptional changes upon PAMP treatment seen in both the A and C genomes of the allotetraploid B. napus. Later responses characterized include callose deposition and lignification at the cell wall, seedling growth inhibition, and PAMP-induced resistance to Pseudomonas syringae and Botrytis cinerea. Furthermore, using these assays, we demonstrated substantial variation in PAMP responses within a collection of diverse B. napus cultivars. The assays reported here could have widespread application in B. napus breeding and mapping programs to improve selection for broad-spectrum disease resistance.
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