Journal
MOLECULAR PLANT PATHOLOGY
Volume 13, Issue 9, Pages 1010-1018Publisher
WILEY
DOI: 10.1111/j.1364-3703.2012.00809.x
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Funding
- Agencia de Promocion Cientifica y Tecnologica (ANPCyT)
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- CONICET
- BBSRC [BBS/E/C/00005203] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/C/00005203] Funding Source: researchfish
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Xanthomonas citri ssp. citri (Xcc) is the causal agent of citrus canker. This bacterium develops a characteristic biofilm on both biotic and abiotic surfaces. A biofilm-deficient mutant was identified in a screening of a transposon mutagenesis library of the Xcc 306 strain constructed using the commercial Tn5 transposon EZ-Tn5 < KAN-2 > Tnp Transposome (Epicentre). Sequence analysis of a mutant obtained in the screening revealed that a single copy of the EZ-Tn5 was inserted at position 446 of hrpM, a gene encoding a putative enzyme involved in glucan synthesis. We demonstrate for the first time that the product encoded by the hrpM gene is involved in beta-1,2-glucan synthesis in Xcc. A mutation in hrpM resulted in no disease symptoms after 4 weeks of inoculation in lemon and grapefruit plants. The mutant also showed reduced ability to swim in soft agar and decreased resistance to H 2 O 2 in comparison with the wild-type strain. All defective phenotypes were restored to wild-type levels by complementation with the plasmid pBBR1-MCS containing an intact copy of the hrpM gene and its promoter. These results indicate that the hrpM gene contributes to Xcc growth and adaptation in its host plant.
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