Journal
MOLECULAR PLANT
Volume 7, Issue 7, Pages 1191-1210Publisher
CELL PRESS
DOI: 10.1093/mp/ssu070
Keywords
hydrogen peroxide; reactive oxygen species; glycolate oxidase; catalase; photorespiration; oxidative signaling
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Funding
- Deutsche Forschungsgemeinschaft (DFG) [MA2379/11-1, EXC 1028]
- Ghent University Multidisciplinary Research Partnership 'Biotechnology for a Sustainable Economy' [01MRB510W]
- Interuniversity Attraction Poles Programme [IUAP P7/29 'MARS']
- Belgian Science Policy Office
- Marie Curie Intra-European Fellowship for Career Development [PIEF-GA-2008-221427]
- Ministry of Higher Education of the Arab Republic of Egypt (MoHE)
- Deutscher Akademischer Austauschdienst (DAAD)
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Hydrogen peroxide (H2O2) operates as a signaling molecule in eukaryotes, but the specificity of its signaling capacities remains largely unrevealed. Here, we analyzed whether a moderate production of H2O2 from two different plant cellular compartments has divergent effects on the plant transcriptome. Arabidopsis thaliana overexpressing glycolate oxidase in the chloroplast (Fahnenstich et al., 2008; Balazadeh et al., 2012) and plants deficient in peroxisomal catalase (Queval et al., 2007; Inze et al., 2012) were grown under non-photorespiratory conditions and then transferred to photorespiratory conditions to foster the production of H2O2 in both organelles. We show that H2O2 originating in a specific organelle induces two types of responses: one that integrates signals independently from the subcellular site of H2O2 production and another that is dependent on the H2O2 production site. H2O2 produced in peroxisomes induces transcripts involved in protein repair responses, while H2O2 produced in chloroplasts induces early signaling responses, including transcription factors and biosynthetic genes involved in production of secondary signaling messengers. There is a significant bias towards the induction of genes involved in responses to wounding and pathogen attack by chloroplastic-produced H2O2, including indolic glucosinolates-, camalexin-, and stigmasterol-biosynthetic genes. These transcriptional (r)esponses were accompanied by the accumulation of 4-methoxy-indol-3-ylmethyl glucosinolate and stigmasterol.
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