Journal
MOLECULAR PLANT
Volume 6, Issue 2, Pages 337-349Publisher
CELL PRESS
DOI: 10.1093/mp/sss100
Keywords
Solanum tuberosum; potato; Arabidopsis thaliana; yuc6-1D; YUCCA; StYUCCA; StTAA; auxin; drought
Categories
Funding
- World Class University Program [R32-10148]
- Ministry of Education, Science, and Technology
- Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ008025, PJ008097]
- National Research Foundation of Korea
- Korean Government (Ministry of Education, Science and Technology) [NRF-2010-359-F00006]
Ask authors/readers for more resources
Indole-3-acetic acid (IAA), a major plant auxin, is produced in both tryptophan-dependent and tryptophan-independent pathways. A major pathway in Arabidopsis thaliana generates IAA in two reactions from tryptophan. Step one converts tryptophan to indole-3-pyruvic acid (IPA) by tryptophan aminotransferases followed by a rate-limiting step converting IPA to IAA catalyzed by YUCCA proteins. We identified eight putative StYUC (Solanum tuberosum YUCCA) genes whose deduced amino acid sequences share 50%70% identity with those of Arabidopsis YUCCA proteins. All include canonical, conserved YUCCA sequences: FATGY motif, FMO signature sequence, and FAD-binding and NADP-binding sequences. In addition, five genes were found with similar to 50% amino acid sequence identity to Arabidopsis tryptophan aminotransferases. Transgenic potato (Solanum tuberosum cv. Jowon) constitutively overexpressing Arabidopsis AtYUC6 displayed high-auxin phenotypes such as narrow downward-curled leaves, increased height, erect stature, and longevity. Transgenic potato plants overexpressing AtYUC6 showed enhanced drought tolerance based on reduced water loss. The phenotype was correlated with reduced levels of reactive oxygen species in leaves. The results suggest a functional YUCCA pathway of auxin biosynthesis in potato that may be exploited to alter plant responses to the environment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available