Journal
MOLECULAR PLANT
Volume 5, Issue 5, Pages 1138-1150Publisher
CELL PRESS
DOI: 10.1093/mp/sss034
Keywords
Arabidopsis thaliana; RNAi; glucosinolate perturbation; proteomics; metabolomics; molecular networks
Categories
Funding
- National Science Foundation [MCB-0845162]
- UF Center for Precollegiate Education Program
- Northeast Forestry University for Excellent Doctoral Dissertation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0845162] Funding Source: National Science Foundation
Ask authors/readers for more resources
To understand plant molecular networks of glucosinolate metabolism, perturbation of aliphatic glucosinolate biosynthesis was established using inducible RNA interference (RNAi) in Arabidopsis. Two RNAi lines were chosen for examining global protein and metabolite changes using complementary proteomics and metabolomics approaches. Proteins involved in metabolism including photosynthesis and hormone metabolism, protein binding, energy, stress, and defense showed marked responses to glucosinolate perturbation. In parallel, metabolomics revealed major changes in the levels of amino acids, carbohydrates, peptides, and hormones. The metabolomics data were correlated with the proteomics results and revealed intimate molecular connections between cellular pathways/processes and glucosinolate metabolism. This study has provided an unprecedented view of the molecular networks of glucosinolate metabolism and laid a foundation towards rationale glucosinolate engineering for enhanced defense and quality.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available