4.7 Review

Glow in the Dark: Fluorescent Proteins as Cell and Tissue-Specific Markers in Plants

Journal

MOLECULAR PLANT
Volume 4, Issue 5, Pages 794-804

Publisher

CELL PRESS
DOI: 10.1093/mp/ssr059

Keywords

Arabidopsis; auxin transport; enhancer trap; FACS; fluorescent proteins; GFP; gene expression markers; live imaging; mathematical modeling; meristem; vascular development

Funding

  1. Natural Sciences and Engineering Research Council of Canada (NSERC)
  2. NSERC CGSM
  3. Centre for Analysis of Genome Evolution and Function (CAGEF)

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Since the hallmark discovery of Aequorea victoria's Green Fluorescent Protein (GFP) and its adaptation for efficient use in plants, fluorescent protein tags marking expression profiles or genuine proteins of interest have been used to recognize plant tissues and cell types, to monitor dynamic cell fate selection processes, and to obtain cell type-specific transcriptomes. Fluorescent tagging enabled visualization in living tissues and the precise recordings of dynamic expression pattern changes. The resulting accurate recording of cell fate acquisition kinetics in space and time has strongly stimulated mathematical modeling of self-organizing feedback mechanisms. In developmental studies, the use of fluorescent proteins has become critical, where morphological markers of tissues, cell types, or differentiation stages are either not known or not easily recognizable. In this review, we focus on the use of fluorescent markers to identify and illuminate otherwise invisible cell states in plant development.

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