Journal
MOLECULAR PHYLOGENETICS AND EVOLUTION
Volume 53, Issue 3, Pages 1049-1054Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ympev.2009.09.003
Keywords
TTR intron 1; Transthyretin; Mecistops cataphractus; Tomistoma schlegelii; Gavialis gangeticus; Alligatoridae; Gavialidae; Crocodylidae; Systematics
Funding
- National Institutes of Health [NIH R01 RR10799]
- NATIONAL CENTER FOR RESEARCH RESOURCES [R01RR010799] Funding Source: NIH RePORTER
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Transthyretin (TTR) is an attractive candidate for use in phylogenetic analysis because it is a short, single-copy nuclear gene with regions that are highly conserved across evolutionarily-divergent organisms from Xenopus laevis to Homo sapiens. To explore its utility as a phylogenetic marker, the complete intron one region (789-805 bp) was sequenced in 22 crocodylian species. Detailed analyses of intron I resolved the three expected lineages, Alligatorids, Crocodylids, and Gavialids, and offered additional evidence for the utility of synapomorphic indels in elucidating higher-level phylogenetic relationships. When used in conjunction with other genetic and morphological data sets, intron 1 should be a valuable tool in the investigation of other closely related taxa. (C) 2009 Elsevier Inc. All rights reserved.
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