Journal
MOLECULAR PHARMACEUTICS
Volume 11, Issue 11, Pages 4270-4279Publisher
AMER CHEMICAL SOC
DOI: 10.1021/mp500473d
Keywords
macrophages; siRNA; microparticles; TNF alpha; aerosol; high-content cell analysis
Funding
- Irish Health Research Board (HRB) [HRB RP/2005/117, PHD/2007/11]
- Science Foundation Ireland (SFI) [SFI SRC/07/B1154]
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The development of small interfering RNA (siRNA) to silence specific genes offers a new means of understanding and treating a range of respiratory diseases, including inflammatory lung disease. The alveolar macrophage (AM) is a key component of the inflammatory process in the lungs, associated with high levels of gene expression in inflammatory lung disease and therefore an attractive target for therapeutic siRNA. Delivery of siRNA to macrophages presents a significant delivery challenge, as fully differentiated alveolar macrophages are difficult to access and transfect. In this study we engineered particles suitable for inhalation that would efficiently transfect macrophages postinhalation. The process for encapsulation of siRNA in poly(lactic-co-glycolic acid) microparticles (MPs) was optimized using a double emulsion technique, and the resulting particles were characterized for size, shape, aerosol characteristics, encapsulation efficiency, and integrity of encapsulated siRNA. The cell uptake of the siRNA-loaded microparticles was determined by flow cytometry, confocal laser scanning microscopy (CLSM), and high-content analysis (HCA) with MPs capable of transfecting up to 55% of cells. Anti-TNFa siRNA-MPs were then prepared to study the functional activity of encapsulated siRNA in LPS-stimulated macrophages as a model of inflammation. The anti-TNFa siRNA-MPs were able to decrease TNFa expression by 45% over 48 h in the differentiated human monocytic cell line THP-1 compared to negligible knockdown using commercial transfection reagents and offered significant, sustained siRNA knockdown of TNFa in primary monocytes for up to 72 h.
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