Journal
SCIENTIA HORTICULTURAE
Volume 184, Issue -, Pages 78-84Publisher
ELSEVIER
DOI: 10.1016/j.scienta.2014.12.031
Keywords
Camellia sinensis; Fluoride; Catechins; Biosynthesis
Categories
Funding
- National Natural Science Foundation of China [31170646]
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Tea plant, Camellia sinensis, can accumulate high levels of fluoride (F); however, it is unclear how F influences secondary metabolism in tea plants. In this study, two tea cultivars seedlings, Fuyunliuhao (FY) and Wuniuzao (WNZ), were cultivated in nutrient solutions containing different concentrations of F for 4 weeks to investigate the effects of F on the content of catechins, the activity of related biosynthetic enzymes in the leaves. The content of tea polyphenols reached to the top at 2 mg L-1 (FY) or 4 mg L-1 (WNZ) and then decreased dose-dependently with the F concentration increasing; the content of catechins increased between 2 and 8 mg L-1 and decreased at 16 mg L-1 significantly comparing with the control; the activity of PAL, C4H, CHI and DFRm in WNZ, DFRm and DFRq in FY increased significantly at 4 mg L-1 F concentration comparing to the control and the activity of ANR in WNZ, C4H in FY decreased dose-dependently with the increasing F concentration, while the activity of PAL, CHI in FY changed little with the F concentration between 0 and 16 mg L-1. These results suggested that F concentrations lower than 4 mg L-1 promote the biosynthesis of catechins in tea plants by up-regulating the activity of related biosynthetic enzymes, whereas F concentrations more than 8 mg L-1 restrain tea plant growth and the synthesis of catechins. The pathways of catechins biosynthesis are tremendously complicated, and further research is required to determine whether F regulates the metabolism of tea catechins by altering other pathways and enzymes in addition to those analyzed in this study. (C) 2014 Elsevier B.V. All rights reserved.
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